Expression of Stro-1, Runx-2, Osterix, and Alp in Alveolar Bone Regeneration Process Following the Administration of Hydroxyapatite Gypsum Puger (HAGP) Scaffold

@#Introduction: Tooth extraction before denture placement could result in trauma and damage to up to 50% of the alveolar bone, inducing bone resorption, and affecting the patient’s quality of life. Hydroxyapatite Gypsum Puger (HAGP) can be used as an alternative to bone graft material which degrades slowly, affecting the proliferation and activity of cells that are responsible for bone tissue engineering. This study aimed to analyze the regeneration mechanism of alveolar bone by administering the HAGP scaffold and observing the Stro-1, Runx-2, Osterix, and ALP expression. Methods: Laboratory experimental research was conducted and we used 150-355µm HAGP scaffold particles, applied in vivo inside alveolar sockets of the rats for 7, 14, and 28 days, followed by immunohistochemical examination of Stro-1, Runx-2, Osterix, and ALP expressions. Results: The HAGP scaffold group showed that the Stro-1 expression was significantly higher than the K(-) group, and the Runx-2 expression increased on day 7 and decreased on day 28 in the HAGP and K(-) groups. Osterix expression increased from day 7, 14, to day 28. The high expression of Osterix on day 28 means it took over the Runx-2 function. In ALP there was a significant increase on day 7. ALP expression was a sign of early osteoblast differentiation and production by cells, this extracellular matrix mineralization is an indicator of the osteogenic process. Conclusion: Alveolar bone regeneration mechanism in rats revealed that the expression of Stro-1, Runx-2, Osterix, and ALP was higher in the HAGP scaffold group compared to the control group on days 7,14, and 28.

Management of short alveolar bone with implant retained splinted crown

@#Implant placement on maxillary posterior region has some limitations because of limited bone height, low bone density, and anatomical consideration involving the floor of the maxillary sinus. A 56-year-old male with a history of hypertension and stroke, consulted for edentulous in teeth 25 and 26 with limited bone height. A short implant for 26 (Ø 4.8 mm; 4 mm) and 25 (Ø 4.1 mm; 10 mm) were placed and splint crown was performed after 6 months.

Bilateral Mandibular Posterior Edentulous rehabilitation for unstable occlusion patient with bilateral attachment retained mandibular removable partial denture

@#A 62-year-old female patient came to the Dental Hospital of Universitas Airlangga to maintain her remaining teeth and make dentures for the missing teeth, and expressed that she expected a high aesthetic result. The patient had lost bilateral posterior mandibular teeth, resulting in unstable occlusion. To stabilize the occlusion, this patient was treated by temporary denture. After 3 months of follow up and confirmed adaptation with the new occlusion, prosthodontic treatment was done consisting of bilateral attachment retained mandibular removable partial denture, aimed at the patient’s expectation of acquiring good aesthetic results and functional rehabilitation.

Aesthetic rehabilitation of the severe bone loss on anterior region using Andrew’s Bridge: A case report

@#A patient with several missing teeth in the anterior region usually experiences severe hard and soft tissue loss, posing a greater challenge for the prosthodontist to treat. This is a case of a 50-year-old female patient who came to the RSGMP UNAIR department of prosthodontics needing to replace the old denture that felt loose and had a change of color to rehabilitate the loss of large residual ridges involving hard and soft tissue. The patient has lost the central and lateral right maxillary incisors with severe hard and soft tissue defect after cyst removal in the region. The patient considered to restore an anterior region with severe hard and tissue loss. Andrew’s Bridge successfully restores an anterior region with severe hard and soft tissue loss.